Journal: Journal of Traditional and Complementary Medicine

Article Title: Gastroprotective effects of Machilus zuihoensis Hayata bark against acidic ethanol-induced gastric ulcer in mice

doi: 10.1016/j.jtcme.2023.05.006

Figure Lengend Snippet: Expression levels of gastric p-NF-κB p65, NF-κB p65, and GAPDH proteins (A), and phosphorylated and total levels of MAPKs and GAPDH (C). PBS treated mice (WT), mice treated with AE only (AE), mice pretreated with 100 mg/kg MZ before AE treatment (MZ100+AE), mice pretreated with 200 mg/kg MZ before AE treatment (MZ200+AE), and mice pretreated with 100 mg/kg CBX before AE treatment (CBX + AE). (B) Ratios of p-NF-κB p65 to GAPDH, (D) ratios of p-ERK to ERK (upper), p-JNK to JNK (middle), and p-p38 to p38 (lower) were calculated. Mice treated with 100 mg/kg MZ (MZ100), 200 mg/kg MZ (MZ200) and 100 mg/kg CBX (CBX), respectively, then treated with AE were underlined. ∗ ( p < 0.05), ∗∗ ( p < 0.01), and ∗∗∗ ( p < 0.001) denote significant differences compared with the AE group.

Article Snippet: Primary antibodies to NF-κB p65 (GTX102090), p38 (GTX110520), and phosphor-p38 (GTX486146) were obtained from GeneTex Inc. (Irvine, CA, USA).

Techniques: Expressing

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm

doi: 10.1155/2021/2502324

Figure Lengend Snippet: CIH regulates CaMKII-dependent MAPK signaling. (a) Apoe −/− mice infused with Ang II and then exposed to CIH or normal conditions. Aortas tissues were collected for RNA sequencing and KEGG analysis. Top 30 of enriched signaling in aortas tissues were shown. (b) Hierarchical clustering and heat map showing the enrichment of the calcium signaling pathway genes in aorta tissues pretreated with normoxia or CIH. Red and blue indicate up- and downregulation, respectively. (c) qRT-PCR analysis of the mRNA expression levels of 11 calcium-related genes in aorta tissues treated with normoxia or CIH ( n = 4). (d) Representative image of Western blot. (e) Quantification of CaMKII, p38, and Jnk from Western blot ( n = 6). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: The following primary antibodies were used to probe targeted proteins: anti-MMP9 (final volume 1 : 500 v / v , GeneTex), anti-MCP1 (1 : 1000 v / v , GeneTex), anti-GAPDH (1 : 5000 v / v , GeneTex), anti-Bax (1 : 500 v / v , GeneTex), anti-Bcl-2 (1 : 500 v / v , GeneTex), anti-caspase-3 (cleaved Asp175, 1 : 500 v / v , GeneTex), anti-p38 (1 : 500 v / v , GeneTex), anti-MMP2 (1 : 500 v / v , Abcam), anti-p-CaMKII (phospho T286, 1 : 1000 v / v , Abcam, Cambridge, MA), anti-CaMKII (1 : 1000 v / v , Abcam), anti-p-p38 (phospho T180, 1 : 1000 v / v , Abcam), anti-Jnk1/2/3 (phospho T183+T183+T221, 1 : 1000 v / v , Abcam), and anti-Jnk1/2/3 (1 : 1000 v / v , Abcam).

Techniques: RNA Sequencing Assay, Quantitative RT-PCR, Expressing, Western Blot

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm

doi: 10.1155/2021/2502324

Figure Lengend Snippet: CIH damages VSMCs through CaMKII-dependent MAPK signaling. The isolated VSMCs from mouse aortas tissues were stimulated with Ang II combined with CIH. An inhibitor of calcium channel (CaMKII-IN-1) was used to treat VSMCs. (a) Representative image of Western blot. (b) Quantification of CaMKII, p38, and Jnk from Western blot ( n = 3). (c) Representative image of Western blot. (d) Quantification of MMP2, MMP9, and MCP1 from Western blot ( n = 3). (e) Representative images showing mtROS levels ( n = 3). (f) Representative image of flow cytometry for testing apoptosis. (g) Quantification of apoptotic cells from flow cytometry ( n = 3). (h) Representative image of Western blot. (i) Quantification of Bax, Bcl-2, and cleaved caspase-3 expressions ( n = 3). ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: The following primary antibodies were used to probe targeted proteins: anti-MMP9 (final volume 1 : 500 v / v , GeneTex), anti-MCP1 (1 : 1000 v / v , GeneTex), anti-GAPDH (1 : 5000 v / v , GeneTex), anti-Bax (1 : 500 v / v , GeneTex), anti-Bcl-2 (1 : 500 v / v , GeneTex), anti-caspase-3 (cleaved Asp175, 1 : 500 v / v , GeneTex), anti-p38 (1 : 500 v / v , GeneTex), anti-MMP2 (1 : 500 v / v , Abcam), anti-p-CaMKII (phospho T286, 1 : 1000 v / v , Abcam, Cambridge, MA), anti-CaMKII (1 : 1000 v / v , Abcam), anti-p-p38 (phospho T180, 1 : 1000 v / v , Abcam), anti-Jnk1/2/3 (phospho T183+T183+T221, 1 : 1000 v / v , Abcam), and anti-Jnk1/2/3 (1 : 1000 v / v , Abcam).

Techniques: Isolation, Western Blot, Flow Cytometry